Identifying targets of the Sox domain protein Dichaete in the Drosophila CNS via targeted expression of dominant negative proteins.

ABSTRACT:

Background

Group B Sox domain transcription factors play important roles in metazoan central nervous system development. They are, however, difficult to study as mutations often have pleiotropic effects and other Sox family members can mask phenotypes due to functional compensation. In Drosophila melanogaster, the Sox gene Dichaete is dynamically expressed in the embryonic CNS, where it is known to have functional roles in neuroblasts and the ventral midline. In this study, we use inducible dominant negative proteins in combination with ChIP, immunohistochemistry and genome-wide expression profiling to further dissect the role of Dichaete in these two tissues.

Results

We generated two dominant negative Dichaete constructs, one lacking a DNA binding domain and the other fused

SUBMITTER: Shen SP 

PROVIDER: S-EPMC3541953 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature