Myosin heavy chain 2A and α-actin expression in human and murine skeletal muscles at feeding; particularly amino acids.

ABSTRACT:

Background

Protein dynamics during non-steady state conditions as feeding are complex. Such studies usually demand combinations of methods to give conclusive information, particularly on myofibrillar proteins with slow turnover. Therefore, time course transcript analyses were evaluated as possible means to monitor changes in myofibrillar biosynthesis in skeletal muscles in conditions with clinical nutrition; i.e. long term exposure of nutrients.

Methods

Muscle tissue from overnight intravenously fed surgical patients were used as a model combined with muscle tissue from starved and refed mice as well as cultured L6 muscle cells. Transcripts of acta 1 (α-actin), mhc2A (myosin) and slc38 a2/Snat 2 (amino acid transporter) were quantified (qPCR) as markers of muscle protein dyn

SUBMITTER: Iresjo BM 

PROVIDER: S-EPMC3542095 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature