High-throughput novel microsatellite marker of faba bean via next generation sequencing.

ABSTRACT:

Background

Faba bean (Vicia faba L.) is an important food legume crop, grown for human consumption globally including in China, Turkey, Egypt and Ethiopia. Although genetic gain has been made through conventional selection and breeding efforts, this could be substantially improved through the application of molecular methods. For this, a set of reliable molecular markers representative of the entire genome is required.

Results

A library with 125,559 putative SSR sequences was constructed and characterized for repeat type and length from a mixed genome of 247 spring and winter sown faba bean genotypes using 454 sequencing. A suit of 28,503 primer pair sequences were designed and 150 were randomly selected for validation. Of these, 94 produced reproducible amplicons that were

SUBMITTER: Yang T 

PROVIDER: S-EPMC3542174 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature