Targeted enrichment of the black cottonwood (Populus trichocarpa) gene space using sequence capture.

ABSTRACT:

Background

High-throughput re-sequencing is rapidly becoming the method of choice for studies of neutral and adaptive processes in natural populations across taxa. As re-sequencing the genome of large numbers of samples is still cost-prohibitive in many cases, methods for genome complexity reduction have been developed in attempts to capture most ecologically-relevant genetic variation. One of these approaches is sequence capture, in which oligonucleotide baits specific to genomic regions of interest are synthesized and used to retrieve and sequence those regions.

Results

We used sequence capture to re-sequence most predicted exons, their upstream regulatory regions, as well as numerous random genomic intervals in a panel of 48 genotypes of the angiosperm tree Populus tricho

SUBMITTER: Zhou L 

PROVIDER: S-EPMC3542275 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature