Elevated levels of circulating microRNA-200 family members correlate with serous epithelial ovarian cancer.

ABSTRACT:

Background

There is a critical need for improved diagnostic markers for high grade serous epithelial ovarian cancer (SEOC). MicroRNAs are stable in the circulation and may have utility as biomarkers of malignancy. We investigated whether levels of serum microRNA could discriminate women with high-grade SEOC from age matched healthy volunteers.

Methods

To identify microRNA of interest, microRNA expression profiling was performed on 4 SEOC cell lines and normal human ovarian surface epithelial cells. Total RNA was extracted from 500 μL aliquots of serum collected from patients with SEOC (n = 28) and age-matched healthy donors (n = 28). Serum microRNA levels were assessed by quantitative RT-PCR following preamplification.

Results

microRNA (miR)-182, miR-200a, miR-200b an

SUBMITTER: Kan CW 

PROVIDER: S-EPMC3542279 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature