Collinearity of protease mutations in HIV-1 samples with high-level protease inhibitor class resistance.

ABSTRACT:

Objectives

To determine whether pan-protease inhibitor (PI)-resistant virus populations are composed predominantly of viruses with resistance to all PIs or of diverse virus populations with resistance to different subsets of PIs.

Methods

We performed deep sequencing of plasma virus samples from nine patients with high-level genotypic and/or phenotypic resistance to all licensed PIs. The nine virus samples had a median of 12 PI resistance mutations by direct PCR Sanger sequencing.

Results

For each of the nine virus samples, deep sequencing showed that each of the individual viruses within a sample contained nearly all of the mutations detected by Sanger sequencing. Indeed, a median of 94.9% of deep sequence reads had each of the PI resistance mutations present as a sin

SUBMITTER: Babrzadeh F 

PROVIDER: S-EPMC3543120 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature