Activation of TRPV4 on dural afferents produces headache-related behavior in a preclinical rat model.

ABSTRACT:

Background

The mechanisms contributing to the pain of migraine are poorly understood although activation of afferent nociceptors in the trigeminovascular system has been proposed as a key event. Prior studies have shown that dural-afferent nociceptors are sensitive to both osmotic and mechanical stimuli. Based on the sensitivity to these stimuli we hypothesized that dural afferents express the osmo/mechano-sensitive channel transient receptor-potential vanilloid 4 (TRPV4).

Methods

These studies used in vitro patch-clamp electrophysiology of trigeminal neurons retrogradely labeled from the dura to examine the functional expression of TRPV4. Additionally, we used a rat headache model in which facial/hind paw allodynia following dural stimulation is measured to determine whethe

SUBMITTER: Wei X 

PROVIDER: S-EPMC3543146 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature