A chemically-defined screening platform reveals behavioral similarities between primary human mesenchymal stem cells and endothelial cells.

ABSTRACT: Chemically defined substrates, which rigorously control protein-surface and cell-surface interactions, can be used to probe the effects of specific biomolecules on cell behavior. Here we combined a chemically-defined, array-based format with automated, time-lapse microscopy to efficiently screen cell-substrate interactions. Self-assembled monolayers (SAMs) of alkanethiolates bearing oligo(ethylene glycol) units and reactive terminal groups were used to present cell adhesion peptides while minimizing non-specific protein interactions. Specifically, we describe rapid fabrication of arrays of 1 mm spots, which present varied densities of the integrin-binding ligand Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP). Results indicate that cell attachment, cell spreading, and proliferation exhibit strong depende

SUBMITTER: Koepsel JT 

PROVIDER: S-EPMC3543770 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature