Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori.

ABSTRACT:

Aim

To identify genes potentially involved in Helicobacter pylori (H. pylori)-induced gastric carcinogenesis.

Methods

GES-1 cells were co-cultured with H. pylori strains isolated from patients with gastric carcinoma (GC, n = 10) or chronic gastritis (CG, n = 10) for in vitro proliferation and apoptosis assays to identify the most and least virulent strains. These two strains were cagA-genotyped and used for further in vivo carcinogenic virulence assays by infecting Mongolian gerbils for 52 wk, respectively; a broth free of H. pylori was lavaged as control. Genomic profiles of GES-1 cells co-cultured with the most and least virulent strains were determined by microarray analysis. The most differentially expressed genes were further verified using quantitative real-time polyme

SUBMITTER: Wang F 

PROVIDER: S-EPMC3544023 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature