Structural mechanism of GAF-regulated σ(54) activators from Aquifex aeolicus.

ABSTRACT: The σ subunits of bacterial RNA polymerase occur in many variant forms and confer promoter specificity to the holopolymerase. Members of the σ(54) family of σ subunits require the action of a 'transcriptional activator' protein to open the promoter and initiate transcription. The activator proteins undergo regulated assembly from inactive dimers to hexamers that are active ATPases. These contact σ(54) directly and, through ATP hydrolysis, drive a conformational change that enables promoter opening. σ(54) activators use several different kinds of regulatory domains to respond to a wide variety of intracellular signals. One common regulatory module, the GAF domain, is used by σ(54) activators to sense small-molecule ligands. The structural basis for GAF domain regulation in σ(54) activators

SUBMITTER: Batchelor JD 

PROVIDER: S-EPMC3544215 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature