PKCδ activation mediates angiogenesis via NADPH oxidase activity in PC-3 prostate cancer cells.

ABSTRACT:

Background

PKCδ is generally known as a pro-apoptotic and anti-proliferative enzyme in human prostate cancer cells.

Methods

Here, we investigated the role of PKCδ on the growth of PC-3 human prostate cancer cells in vivo and in vitro.

Results

We found that sustained treatment with a specific PKCδ activator (ψδ receptor for active C kinase, ψδRACK) increased growth of PC-3 xenografts. There was increased levels of HIF-1α, vascular endothelial growth factor and CD31-positive cells in PC-3 xenografts, representative of increased tumor angiogenesis. Mechanistically, PKCδ activation increased the levels of reactive oxygen species (ROS) by binding to and phosphorylating NADPH oxidase, which induced its activity. Also, PKCδ-induced activation of NADPH oxidase increased the l

SUBMITTER: Kim J 

PROVIDER: S-EPMC3544470 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature