Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins.

ABSTRACT:

Background

Conventional fluorescent proteins, such as GFP, its derivatives and flavin mononucleotide based fluorescent proteins (FbFPs) are often used as fusion tags for detecting recombinant proteins during cultivation. These reporter tags are state-of-the-art; however, they have some drawbacks, which can make on-line monitoring challenging. It is discussed in the literature that the large molecular size of proteins of the GFP family may stress the host cell metabolism during production. In addition, fluorophore formation of GFP derivatives is oxygen-dependent resulting in a lag-time between expression and fluorescence detection and the maturation of the protein is suppressed under oxygen-limited conditions. On the contrary, FbFPs are also applicable in an oxygen-limited or even a

SUBMITTER: Siepert EM 

PROVIDER: S-EPMC3544578 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature