An individual urinary proteome analysis in normal human beings to define the minimal sample number to represent the normal urinary proteome.

ABSTRACT:

Unlabelled

Background

The urinary proteome has been widely used for biomarker discovery. A urinary proteome database from normal humans can provide a background for discovery proteomics and candidate proteins/peptides for targeted proteomics. Therefore, it is necessary to define the minimum number of individuals required for sampling to represent the normal urinary proteome.

Methods

In this study, inter-individual and inter-gender variations of urinary proteome were taken into consideration to achieve a representative database. An individual analysis was performed on overnight urine samples from 20 normal volunteers (10 males and 10 females) by 1DLC/MS/MS. To obtain a representative result of each sample, a replicate 1DLCMS/MS analysis was performed. The minimal samp

SUBMITTER: Liu X 

PROVIDER: S-EPMC3544588 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature