Quantitative 3-dimensional corneal imaging in vivo using a modified HRT-RCM confocal microscope.

ABSTRACT:

Purpose

The purpose of this study was to develop and test hardware and software modifications to allow quantitative full-thickness corneal imaging using the Heidelberg Retina Tomograph (HRT) Rostock Corneal Module.

Methods

A personal computer-controlled motor drive with positional feedback was integrated into the system to allow automated focusing through the entire cornea. The left eyes of 10 New Zealand white rabbits were scanned from endothelium to epithelium. Image sequences were read into a custom-developed program for depth calculation and measurement of sublayer thicknesses. Three-dimensional visualizations were also generated using Imaris. In 6 rabbits, stack images were registered, and depth-dependent counts of keratocyte nuclei were made using Metamorph.

Results

SUBMITTER: Petroll WM 

PROVIDER: S-EPMC3546130 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature