Wnt pathway activation predicts increased risk of tumor recurrence in patients with stage I nonsmall cell lung cancer.

ABSTRACT:

Objective

To determine the incidence of Wnt pathway activation in patients with stage I NSCLC and its influence on lung cancer recurrence.

Background

Despite resection, the 5-year recurrence with localized stage I nonsmall cell lung cancer (NSCLC) is 18.4%-24%. Aberrant Wnt signaling activation plays an important role in a wide variety of tumor types. However, there is not much known about the role the Wnt pathway plays in patients with stage I lung cancer.

Methods

Tumor and normal lung tissues from 55 patients following resection for stage I NSCLC were subjected to glutathione S-transferase (GST) E-cadherin pulldown and immunoblot analysis to assess levels of uncomplexed β-catenin, a reliable measure of Wnt signaling activation. The β-catenin gene was also screened f

SUBMITTER: Shapiro M 

PROVIDER: S-EPMC3546156 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature