An organotypic uniaxial strain model using microfluidics.

ABSTRACT: Traumatic brain injuries are the leading cause of disability each year in the US. The most common and devastating consequence is the stretching of axons caused by shear deformation that occurs during rotational acceleration of the brain during injury. The injury effects on axonal molecular and functional events are not fully characterized. We have developed a strain injury model that maintains the three dimensional cell architecture and neuronal networks found in vivo with the ability to visualize individual axons and their response to a mechanical injury. The advantage of this model is that it can apply uniaxial strains to axons that make functional connections between two organotypic slices and injury responses can be observed in real-time and over long term. This uniaxial strain model w

SUBMITTER: Dolle JP 

PROVIDER: S-EPMC3546521 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature