Modelling translation initiation under the influence of sRNA.

ABSTRACT: Bacterial small non-coding RNA (sRNA) plays an important role in post-transcriptional gene regulation. Although the number of annotated sRNA is steadily increasing, their functional characterization is still lagging behind. Various computational strategies for finding sRNA−mRNA interactions, and thus putative sRNA targets, were developed. Most of them suffer from a high false positive rate. Here, we present a qualitative model to simulate the effect of an sRNA on the translation initiation of a potential target. Information about the ribosome−mRNA interaction, sRNA−mRNA interaction and expression information from deep sequencing experiments is integrated to calculate the change in translation initiation complex formation, as a proxy for translational activity. This model can be used to pos

SUBMITTER: Amman F 

PROVIDER: S-EPMC3546686 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature