Next generation sequencing and comparative analyses of Xenopus mitogenomes.

ABSTRACT:

Background

Mitochondrial genomes comprise a small but critical component of the total DNA in eukaryotic organisms. They encode several key proteins for the cell's major energy producing apparatus, the mitochondrial respiratory chain. Additionally, their nucleotide and amino acid sequences are of great utility as markers for systematics, molecular ecology and forensics. Their characterization through nucleotide sequencing is a fundamental starting point in mitogenomics. Methods to amplify complete mitochondrial genomes rapidly and efficiently from microgram quantities of tissue of single individuals are, however, not always available. Here we validate two approaches, which combine long-PCR with Roche 454 pyrosequencing technology, to obtain two complete mitochondrial genomes from in

SUBMITTER: Lloyd RE 

PROVIDER: S-EPMC3546946 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature