Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD).

ABSTRACT:

Background

The identification of new virus strains is important for the study of infectious disease, but current (or existing) molecular biology methods are limited since the target sequence must be known to design genome-specific PCR primers. Thus, we developed a new method for the discovery of unknown viruses based on the cDNA--random amplified polymorphic DNA (cDNA-RAPD) technique. Getah virus, belonging to the family Togaviridae in the genus Alphavirus, is a mosquito-borne enveloped RNA virus that was identified using the Virus-Discovery-cDNA RAPD (VIDISCR) method.

Results

A novel Getah virus was identified by VIDISCR from suckling mice exposed to mosquitoes (Aedes albopictus) collected in Yunnan Province, China. The non-structural protein gene, nsP3, the structural prot

SUBMITTER: Hu T 

PROVIDER: S-EPMC3547691 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature