Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patients.

ABSTRACT:

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Background

Dengue fever is the most important arthropod born viral disease of public health significance. Although most patients suffer only from flu-like symptoms, a small group of patient experiences more severe forms of the disease. To contribute to a better understanding of its pathogenesis this study aims to identify proteins differentially expressed in a pool of five viremic plasma from severe dengue patients relative to a pool of five non-severe dengue patients.

Results

The use of Isotope Coded Protein Labeling (ICPLTM) to analyze plasma depleted of twenty high-abundance proteins allowed for the identification of 51 differentially expressed proteins, which were characterized by mass spectrometry. Using quantitative ELISA, three of these proteins (Le

SUBMITTER: Fragnoud R 

PROVIDER: S-EPMC3547721 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature