Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.

ABSTRACT: The stabilization of the replisome complex is essential in order to achieve highly processive DNA replication and preserve genomic integrity. Conversely, it would also be advantageous for the cell to abrogate replisome functions to prevent inappropriate replication when fork progression is adversely perturbed. However, such mechanisms remain elusive. Here we report that replicative DNA polymerases and helicases, the major components of the replisome, are degraded in concert in the absence of Swi1, a subunit of the replication fork protection complex. In sharp contrast, ORC and PCNA, which are also required for DNA replication, were stably maintained. We demonstrate that this degradation of DNA polymerases and helicases is dependent on the ubiquitin-proteasome system, in which the SCF(Pof3)

SUBMITTER: Roseaulin LC 

PROVIDER: S-EPMC3547854 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature