Assessing bioenergetic function in response to oxidative stress by metabolic profiling.

ABSTRACT: It is now clear that mitochondria are an important target for oxidative stress in a broad range of pathologies, including cardiovascular disease, diabetes, neurodegeneration, and cancer. Methods for assessing the impact of reactive species on isolated mitochondria are well established but constrained by the need for large amounts of material to prepare intact mitochondria for polarographic measurements. With the availability of high-resolution polarography and fluorescence techniques for the measurement of oxygen concentration in solution, measurements of mitochondrial function in intact cells can be made. Recently, the development of extracellular flux methods to monitor changes in oxygen concentration and pH in cultures of adherent cells in multiple-sample wells simultaneously has greatl

SUBMITTER: Dranka BP 

PROVIDER: S-EPMC3548422 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature