Strand-specific RNA-seq reveals widespread occurrence of novel cis-natural antisense transcripts in rice.

ABSTRACT:

Background

Cis-natural antisense transcripts (cis-NATs) are RNAs transcribed from the antisense strand of a gene locus, and are complementary to the RNA transcribed from the sense strand. Common techniques including microarray approach and analysis of transcriptome databases are the major ways to globally identify cis-NATs in various eukaryotic organisms. Genome-wide in silico analysis has identified a large number of cis-NATs that may generate endogenous short interfering RNAs (nat-siRNAs), which participate in important biogenesis mechanisms for transcriptional and post-transcriptional regulation in rice. However, the transcriptomes are yet to be deeply sequenced to comprehensively investigate cis-NATs.

Results

We applied high-throughput strand-specific complementary DNA s

SUBMITTER: Lu T 

PROVIDER: S-EPMC3549290 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature