Expression profiles of muscle disease-associated genes and their isoforms during differentiation of cultured human skeletal muscle cells.

ABSTRACT:

Background

The formation of contractile myofibrils requires the stepwise onset of expression of muscle specific proteins. It is likely that elucidation of the expression patterns of muscle-specific sarcomeric proteins is important to understand muscle disorders originating from defects in contractile sarcomeric proteins.

Methods

We investigated the expression profile of a panel of sarcomeric components with a focus on proteins associated with a group of congenital disorders. The analyses were performed in cultured human skeletal muscle cells during myoblast proliferation and myotube development.

Results

Our culture technique resulted in the development of striated myotubes and the expression of adult isoforms of the sarcomeric proteins, such as fast TnI, fast TnT, adu

SUBMITTER: Abdul-Hussein S 

PROVIDER: S-EPMC3549291 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature