Enhanced anti-HIV efficacy of indinavir after inclusion in CD4-targeted lipid nanoparticles.

ABSTRACT:

Background

Combination drug therapy has reduced plasma HIV to undetectable levels; however, drug-sensitive virus persists in patients' lymphoid tissue. We have reported significant lymphoid tissue drug localization with indinavir-associated lipid nanoparticles (LNPs). Our current objective is to evaluate whether additional enhancement is achievable by targeting these particles to CD4-HIV host cells.

Methods

We characterized 2 peptide-coated (CD4-BP2 and CD4-BP4) drug-associated LNPs and demonstrated CD4-cell specificity. Drug-associated LNPs expressing polyethyleneglycol were exposed on HIV-2-infected cells under dynamic conditions that emulated lymph node physiology for 15, 30, and 60 minutes at concentrations from 0 to 25 μM and evaluated for antiviral activity and cell-as

SUBMITTER: Endsley AN 

PROVIDER: S-EPMC3551348 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature