FK506 biosynthesis is regulated by two positive regulatory elements in Streptomyces tsukubaensis.

ABSTRACT:

Background

FK506 (Tacrolimus) is an important immunosuppressant, produced by industrial biosynthetic processes using various Streptomyces species. Considering the complex structure of FK506, it is reasonable to expect complex regulatory networks controlling its biosynthesis. Regulatory elements, present in gene clusters can have a profound influence on the final yield of target product and can play an important role in development of industrial bioprocesses.

Results

Three putative regulatory elements, namely fkbR, belonging to the LysR-type family, fkbN, a large ATP-binding regulator of the LuxR family (LAL-type) and allN, a homologue of AsnC family regulatory proteins, were identified in the FK506 gene cluster from Streptomyces tsukubaensis NRRL 18488, a progenitor of indus

SUBMITTER: Goranovic D 

PROVIDER: S-EPMC3551636 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature