Exposure to ionizing radiation induced persistent gene expression changes in mouse mammary gland.

ABSTRACT:

Background

Breast tissue is among the most sensitive tissues to the carcinogenic actions of ionizing radiation and epidemiological studies have linked radiation exposure to breast cancer. Currently, molecular understanding of radiation carcinogenesis in mammary gland is hindered due to the scarcity of in vivo long-term follow up data. We undertook this study to delineate radiation-induced persistent alterations in gene expression in mouse mammary glands 2-month after radiation exposure.

Methods

Six to eight week old female C57BL/6J mice were exposed to 2 Gy of whole body γ radiation and mammary glands were surgically removed 2-month after radiation. RNA was isolated and microarray hybridization performed for gene expression analysis. Ingenuity Pathway Analysis (IPA) was used

SUBMITTER: Datta K 

PROVIDER: S-EPMC3551737 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature