Evidence for a glucocorticoid receptor beta splice variant in the rat and its physiological regulation in liver.

ABSTRACT: Glucocorticoids are important regulators of metabolism and immune function. Synthetic glucocorticoids are extensively used for immunosuppression/anti-inflammatory therapy. Since the glucocorticoid receptor (GR) is central to most hormone effects; its in vivo regulation will influence hormone/drug action. An alternative splice variant, GRβ, is present in humans and may function as a dominant negative regulator of GR transcriptional activity. Recently, a similar splice variant was reported in mouse, although the mechanism of alternative splicing differs from that in humans. We present evidence that a splice variant of GR with an alternative C-terminus also occurs in the rat by a mechanism of intron inclusion. A highly quantitative qRT-PCR assay for the simultaneous measurement of both splice

SUBMITTER: DuBois DC 

PROVIDER: S-EPMC3552070 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature