A novel FoxD3 gene trap line reveals neural crest precursor movement and a role for FoxD3 in their specification.

ABSTRACT: Neural crest cells migrate extensively and contribute to diverse derivatives, including the craniofacial skeleton, peripheral neurons and glia, and pigment cells. Although several transgenic lines label neural crest subpopulations, few are suited for studying early events in neural crest development. Here, we present a zebrafish gene/protein trap line gt(foxd3-citrine)(ct110a) that expresses a Citrine fusion protein with FoxD3, a transcription factor expressed in premigratory and migrating neural crest cells. In this novel line, citrine expression exactly parallels endogenous foxd3 expression. High-resolution time-lapse imaging reveals the dynamic phases of precursor and migratory neural crest cell movements from the neural keel stage to times of active cell migration. In addition, Cre-rec

SUBMITTER: Hochgreb-Hagele T 

PROVIDER: S-EPMC3553214 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature