Intracellularly induced cyclophilins play an important role in stress adaptation and virulence of Brucella abortus.

ABSTRACT: Brucella is an intracellular bacterial pathogen that causes the worldwide zoonotic disease brucellosis. Brucella virulence relies on its ability to transition to an intracellular lifestyle within host cells. Thus, this pathogen must sense its intracellular localization and then reprogram gene expression for survival within the host cell. A comparative proteomic investigation was performed to identify differentially expressed proteins potentially relevant for Brucella intracellular adaptation. Two proteins identified as cyclophilins (CypA and CypB) were overexpressed in the intracellular environment of the host cell in comparison to laboratory-grown Brucella. To define the potential role of cyclophilins in Brucella virulence, a double-deletion mutant was constructed and its resulting phenot

SUBMITTER: Roset MS 

PROVIDER: S-EPMC3553818 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature