Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system.

ABSTRACT: Targeted genome engineering has become an important research area for diverse disciplines, with site-specific recombinases (SSRs) being among the most popular genome engineering tools. Their ability to trigger excision, integration, inversion and translocation has made SSRs an invaluable tool to manipulate DNA in vitro and in vivo. However, sophisticated strategies that combine different SSR systems are ever increasing. Hence, the demand for additional precise and efficient recombinases is dictated by the increasing complexity of the genetic studies. Here, we describe a novel site-specific recombination system designated Vika/vox. Vika originates from a degenerate bacteriophage of Vibrio coralliilyticus and shares low sequence similarity to other tyrosine recombinases, but functionally car

SUBMITTER: Karimova M 

PROVIDER: S-EPMC3553980 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature