The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets.

ABSTRACT: Animal miRNAs silence the expression of mRNA targets through translational repression, deadenylation and subsequent mRNA degradation. Silencing requires association of miRNAs with an Argonaute protein and a GW182 family protein. In turn, GW182 proteins interact with poly(A)-binding protein (PABP) and the PAN2-PAN3 and CCR4-NOT deadenylase complexes. These interactions are required for the deadenylation and decay of miRNA targets. Recent studies have indicated that miRNAs repress translation before inducing target deadenylation and decay; however, whether translational repression and deadenylation are coupled or represent independent repressive mechanisms is unclear. Another remaining question is whether translational repression also requires GW182 proteins to interact with both PABP and de

SUBMITTER: Huntzinger E 

PROVIDER: S-EPMC3553986 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature