Sequencing and de novo assembly of the western tarnished plant bug (Lygus hesperus) transcriptome.

ABSTRACT:

Background

Mirid plant bugs (Hemiptera: Miridae) are economically important insect pests of many crops worldwide. The western tarnished plant bug Lygus hesperus Knight is a pest of cotton, alfalfa, fruit and vegetable crops, and potentially of several emerging biofuel and natural product feedstocks in the western US. However, little is known about the underlying molecular genetics, biochemistry, or physiology of L. hesperus, including their ability to survive extreme environmental conditions.

Methodology/principal findings

We used 454 pyrosequencing of a normalized adult cDNA library and de novo assembly to obtain an adult L. hesperus transcriptome consisting of 1,429,818 transcriptomic reads representing 36,131 transcript isoforms (isotigs) that correspond to 19,742 genes.

SUBMITTER: Hull JJ 

PROVIDER: S-EPMC3554660 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature