Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.

ABSTRACT:

Background

Tuberculosis (TB) remains a global threat in the 21st century. Traditional studies of the disease are focused on the single pathogen Mycobacterium tuberculosis. Recent studies have revealed associations of some diseases with an imbalance in the microbial community. Characterization of the TB microbiota could allow a better understanding of the disease.

Methodology/principal findings

Here, the sputum microbiota in TB infection was examined by using 16S rRNA pyrosequencing. A total of 829,873 high-quality sequencing reads were generated from 22 TB and 14 control sputum samples. Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, and Fusobacteria were the five major bacterial phyla recovered, which together composed over 98% of the microbial community. Proteob

SUBMITTER: Cheung MK 

PROVIDER: S-EPMC3554703 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature