Primarily screening and analyzing ESTs differentially expressed in rats' primary liver cancer.

ABSTRACT:

Objective

To screen and analyze key express sequence tags (ESTs) which were differentially displayed in every period of SD rats' primary hepatic carcinoma and reveal the molecular mechanism of carcinogenesis.

Methods

Using diethylnitrosamine (DENA) as a cancerigenic agent, animal models with different phases of primary hepatic cancer were constructed in SD rats. Rats were respectively sacrificed at d 14, d 28, d 56, d 77, d 105 and d 112 after the rats received DENA by gavage, then the livers were harvested. One part of the livers was classified according to their pathological changes, while the other was reserved for molecular mechanism studies on hepatocarcinogenesis. The differentially expressed genes were isolated from both normal and morbid tissues by mRNA differential

SUBMITTER: Liu D 

PROVIDER: S-EPMC3555298 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature