Tomographic sensing and localization of fluorescently labeled circulating cells in mice in vivo.

ABSTRACT: Sensing and enumeration of specific types of circulating cells in small animals is an important problem in many areas of biomedical research. Microscopy-based fluorescence in vivo flow cytometry methods have been developed previously, but these are typically limited to sampling of very small blood volumes, so that very rare circulating cells may escape detection. Recently, we described the development of a 'diffuse fluorescence flow cytometer' (DFFC) that allows sampling of much larger blood vessels and therefore circulating blood volumes in the hindlimb, forelimb or tail of a mouse. In this work, we extend this concept by developing and validating a method to tomographically localize circulating fluorescently labeled cells in the cross section of a tissue simulating optical flow phantom a

SUBMITTER: Zettergren E 

PROVIDER: S-EPMC3555500 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature