Development of a method for the measurement of primary cilia length in 3D.

ABSTRACT:

Unlabelled

Background

Primary cilia length is an important measure of cell and tissue function. While accurate length measurements can be calculated from cells in 2D culture, measurements in tissue or 3D culture are inherently difficult due to optical distortions. This study uses a novel combination of image processing techniques to rectify optical distortions and accurately measure cilia length from 3D images.

Methods

Point spread functions and experimental resolutions were calculated from subresolution microspheres embedded in 3D agarose gels for both wide-field fluorescence and confocal laser scanning microscopes. The degree of axial smearing and spherical aberration was calculated from xy:xz diameter ratios of 3D image data sets of 4 μm microspheres that had unde

SUBMITTER: Saggese T 

PROVIDER: S-EPMC3555708 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature