The miRNA profile of human pancreatic islets and beta-cells and relationship to type 2 diabetes pathogenesis.

ABSTRACT: Recent advances in the understanding of the genetics of type 2 diabetes (T2D) susceptibility have focused attention on the regulation of transcriptional activity within the pancreatic beta-cell. MicroRNAs (miRNAs) represent an important component of regulatory control, and have proven roles in the development of human disease and control of glucose homeostasis. We set out to establish the miRNA profile of human pancreatic islets and of enriched beta-cell populations, and to explore their potential involvement in T2D susceptibility. We used Illumina small RNA sequencing to profile the miRNA fraction in three preparations each of primary human islets and of enriched beta-cells generated by fluorescence-activated cell sorting. In total, 366 miRNAs were found to be expressed (i.e. >100 cumulat

SUBMITTER: van de Bunt M 

PROVIDER: S-EPMC3555946 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature