Mammalian Clusterin associated protein 1 is an evolutionarily conserved protein required for ciliogenesis.

ABSTRACT:

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Background

Clusterin associated protein 1 (CLUAP1) was initially characterized as a protein that interacts with clusterin, and whose gene is frequently upregulated in colon cancer. Although the consequences of these observations remain unclear, research of CLUAP1 homologs in C. elegans and zebrafish indicates that it is needed for cilia assembly and maintenance in these models. To begin evaluating whether Cluap1 has an evolutionarily conserved role in cilia in mammalian systems and to explore the association of Cluap1 with disease pathogenesis and developmental abnormalities, we generated Cluap1 mutant mice.

Methods

Cluap1 mutant embryos were generated and examined for gross morphological and anatomical defects using light microscopy. Reverse transcription

SUBMITTER: Pasek RC 

PROVIDER: S-EPMC3556011 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature