Development of a novel two-dimensional directed differentiation system for generation of cardiomyocytes from human pluripotent stem cells.

ABSTRACT:

Background

Human pluripotent stem cells (hPSCs) hold great promise for treating ischemic heart disease. However, current protocols for differentiating hPSCs either result in low yields or require expensive cytokines.

Methods

Here we developed a novel two dimensional (2D) stepwise differentiation system that generates a high yield of cardiomyocytes (CMs) from hPSCs without using special cytokines. Initially, undifferentiated hPSCs were transferred onto Matrigel-coated plates without forming embryoid bodies (EBs) for a few days and were cultured in bFGF-depleted human embryonic stem cells (hESCs) medium. When linear cell aggregation appeared in the margins of the hPSC colonies, the medium was changed to DMEM supplemented with 10% fetal bovine serum (FBS). Thereafter when cell

SUBMITTER: Moon SH 

PROVIDER: S-EPMC3556195 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature