Graded or threshold response of the tet-controlled gene expression: all depends on the concentration of the transactivator.

ABSTRACT:

Background

Currently, the step-wise integration of tet-dependent transactivator and tet-responsive expression unit is considered to be the most promising tool to achieve stable tet-controlled gene expression in cell populations. However, disadvantages of this strategy for integration into primary cells led us to develop an "All-In-One" vector system, enabling simultaneous integration of both components. The effect on tet-controlled gene expression was analyzed for retroviral "All-In-One" vectors expressing the M2-transactivator either under control of a constitutive or a new type of autoregulated promoter.

Results

Determination of luciferase activity in transduced cell populations indicated improvement of the dynamic range of gene expression for the autoregulated system. Fur

SUBMITTER: Heinz N 

PROVIDER: S-EPMC3556329 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature