De novo transcriptome characterization of Vitis vinifera cv. Corvina unveils varietal diversity.

ABSTRACT:

Background

Plants such as grapevine (Vitis spp.) display significant inter-cultivar genetic and phenotypic variation. The genetic components underlying phenotypic diversity in grapevine must be understood in order to disentangle genetic and environmental factors.

Results

We have shown that cDNA sequencing by RNA-seq is a robust approach for the characterization of varietal diversity between a local grapevine cultivar (Corvina) and the PN40024 reference genome. We detected 15,161 known genes including 9463 with novel splice isoforms, and identified 2321 potentially novel protein-coding genes in non-annotated or unassembled regions of the reference genome. We also discovered 180 apparent private genes in the Corvina genome which were missing from the reference genome.

Concl

SUBMITTER: Venturini L 

PROVIDER: S-EPMC3556335 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature