Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains.

ABSTRACT: Transcription activator-like effectors are sequence-specific DNA-binding proteins that harbour modular, repetitive DNA-binding domains. Transcription activator-like effectors have enabled the creation of customizable designer transcriptional factors and sequence-specific nucleases for genome engineering. Here we report two improvements of the transcription activator-like effector toolbox for achieving efficient activation and repression of endogenous gene expression in mammalian cells. We show that the naturally occurring repeat-variable diresidue Asn-His (NH) has high biological activity and specificity for guanine, a highly prevalent base in mammalian genomes. We also report an effective transcription activator-like effector transcriptional repressor architecture for targeted inhibition

SUBMITTER: Cong L 

PROVIDER: S-EPMC3556390 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature