Digital transcriptome profiling of normal and glioblastoma-derived neural stem cells identifies genes associated with patient survival.

ABSTRACT:

Background

Glioblastoma multiforme, the most common type of primary brain tumor in adults, is driven by cells with neural stem (NS) cell characteristics. Using derivation methods developed for NS cells, it is possible to expand tumorigenic stem cells continuously in vitro. Although these glioblastoma-derived neural stem (GNS) cells are highly similar to normal NS cells, they harbor mutations typical of gliomas and initiate authentic tumors following orthotopic xenotransplantation. Here, we analyzed GNS and NS cell transcriptomes to identify gene expression alterations underlying the disease phenotype.

Methods

Sensitive measurements of gene expression were obtained by high-throughput sequencing of transcript tags (Tag-seq) on adherent GNS cell lines from three glioblastoma ca

SUBMITTER: Engstrom PG 

PROVIDER: S-EPMC3556652 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature