High-throughput interrogation of ligand binding mode using a fluorescence-based assay.

ABSTRACT: Probing the pocket: A high-throughput fluorescence-based thermal shift (FTS) assay utilized different forms of a protein (in gray) to establish the binding mode of a ligand (see picture). The assay serves in the rapid evaluation of structure-activity binding-mode relationships for a series of ligands of Plk1, an important target of anticancer therapy.

SUBMITTER: Sledz P 

PROVIDER: S-EPMC3556687 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature