Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation.

ABSTRACT:

Background

Fluorescence loss in photobleaching (FLIP) is a widely used imaging technique, which provides information about protein dynamics in various cellular regions. In FLIP, a small cellular region is repeatedly illuminated by an intense laser pulse, while images are taken with reduced laser power with a time lag between the bleaches. Despite its popularity, tools are lacking for quantitative analysis of FLIP experiments. Typically, the user defines regions of interest (ROIs) for further analysis which is subjective and does not allow for comparing different cells and experimental settings.

Results

We present two complementary methods to detect and quantify protein transport and aggregation in living cells from FLIP image series. In the first approach, a stretched expone

SUBMITTER: Wustner D 

PROVIDER: S-EPMC3557157 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature