Impact of the antiproliferative agent ciclopirox olamine treatment on stem cells proteome.

ABSTRACT:

Aim

To investigate the proteome changes of stem cells due to ciclopirox olamine (CPX) treatment compared to control and retinoic acid treated cells.

Methods

Stem cells (SCs) are cells, which have the ability to continuously divide and differentiate into various other kinds of cells. Murine embryonic stem cells (ESCs) and multipotent adult germline stem cells (maGSCs) were treated with CPX, which has been shown to have an antiproliferative effect on stem cells, and compared to stem cells treated with retinoic acid (RA), which is known to have a differentiating effect on stem cells. Classical proteomic techniques like 2-D gel electrophoresis and differential in-gel electrophoresis (DIGE) were used to generate 2D protein maps from stem cells treated with RA or CPX as well as fr

SUBMITTER: Dihazi GH 

PROVIDER: S-EPMC3557350 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature