Increased dietary intake of vitamin A promotes aortic valve calcification in vivo.

ABSTRACT:

Objective

Calcific aortic valve disease (CAVD) is a major public health problem with no effective treatment available other than surgery. We previously showed that mature heart valves calcify in response to retinoic acid (RA) treatment through downregulation of the SRY transcription factor Sox9. In this study, we investigated the effects of excess vitamin A and its metabolite RA on heart valve structure and function in vivo and examined the molecular mechanisms of RA signaling during the calcification process in vitro.

Methods and results

Using a combination of approaches, we defined calcific aortic valve disease pathogenesis in mice fed 200 IU/g and 20 IU/g of retinyl palmitate for 12 months at molecular, cellular, and functional levels. We show that mice fed excess vitamin

SUBMITTER: Huk DJ 

PROVIDER: S-EPMC3557503 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature