Pathogenicity of exopolysaccharide-producing Actinomyces oris isolated from an apical abscess lesion.

ABSTRACT:

Aim

To demonstrate a capacity for producing exopolysaccharides (EPSs) and an ability to form biofilm on abiotic materials of Actinomyces oris strain K20.

Methodology

The productivity of EPSs and the ability to form biofilm of strain K20 were evaluated by measuring viscosity of spent culture media and by scanning electron microscopy (SEM) and the biofilm assay on microtitre plates, respectively. High-performance liquid chromatography was used to determine the chemical composition of the viscous materials. To examine the role of the viscous materials attributable to the pathogenicity in this organism, the ability of strain K20 to induce abscess formation was compared in mice to that of ATCC 27044.

Results

The viscosity of the spent culture media of K20 was significantly

SUBMITTER: Yamane K 

PROVIDER: S-EPMC3557718 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature