Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase.

ABSTRACT:

Background

Laccases are blue multi-copper oxidases and catalyze the oxidation of phenolic and non-phenolic compounds. There is considerable interest in using these enzymes for dye degradation as well as for synthesis of aromatic compounds. Laccases are produced at relatively low levels and, sometimes, as isozymes in the native fungi. The investigation of properties of individual enzymes therefore becomes difficult. The goal of this study was to over-produce a previously reported laccase from Cyathus bulleri using the well-established expression system of Pichia pastoris and examine and compare the properties of the recombinant enzyme with that of the native laccase.

Results

In this study, complete cDNA encoding laccase (Lac) from white rot fungus Cyathus bulleri was amplifie

SUBMITTER: Garg N 

PROVIDER: S-EPMC3558336 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature